One interesting study is called, "The absence of SV40 large T-antigen expression in human mesothelioma cell lines." With Pilatte Y, Vivo C, Renier A, Kheuang L, Greffard A, Jaurand MC - Am J Respir your Mol Biol. December 2000, 23 (6) :788-93. - INSERM, Creteil, France. Here is an excerpt: "Abstract - Simian virus (SV) 40 and SV40-like DNA sequences has recently been detected in several types of human tumors, including malignant mesothelioma. However, the presence of SV40 DNA sequences are not sufficient to explain the possible role in tumor progression because viral proteins to be expressed and ultimately destroy the function of the relevant cell proteins, such as p53 and PRB. In this study we examined the SV40 large T antigen (SV40 Tag) protein expression in mesothelioma cell lines, established in our laboratory, by Western blotting, immunoprecipitation immunocytochemistry, and use the Tag-specific antibody mouse monoclonal (mAbs) Ab-1 (or 419 Pab). Through Western blotting of cell extracts, no mesothelioma cell lines expressed detectable amounts of SV40 Tag. however, we found that the Ab-1 and Pab -101, another 40 SV Tag-specific mAb, can produce a false positive signal due to the fact that both antibody preparations were contaminated by proteins of similar size (90 kD) as SV40 Tag and reacted with secondary horseradish peroxidase-conjugated various antimouse immunoglobulin Gs tested . study shows that Immunodetection of SV40 Tag protein may be confusing because Taglike contaminating proteins can bind to specific cell structures, resulting in false-positive signals. "
Another study is called, "Pseudomesotheliomatous angiosarcoma: pleuropulmonary lesion simulating malignant pleural Mesothelioma" by G. Falconieri, R. Bussani1, M. Mirra, M. Zanella - Histopathology Volume 30, Issue 5, pages 419-424, May 1997. Here is an excerpt: "We report two cases of angiosarcoma autopsy confirmed in adult men, presenting as pleuropulmonary tumor spread and simulation of malignant mesothelioma. Both lesions grow along the surface of serous and characterized by variable thick rinds wrap lung tissue. Showed diffuse lung parenchyma, red dark, subpleural consolidation and cavitation few. Histologically, the lesions composed by atypical spindle and polygonal, epithelioid cells showed differentiation vascular basis and showed a strong positive for factor VIII, CD31 CD34 and vimentin. angiosarcoma We conclude that may be present with greater involvement or exclusive of the pleura and peripheral lung and that it should be added to the list of tumors can simulate malignant mesothelioma. "
Another study is called, "D2-40: A Reliable Marker in Diagnosis Pleural Mesothelioma" by Annette M. Müllera, Folker E. Frankeb, Klaus-Michael Müllera - Institute of Pathology, 'Bergmannsheil' BG Clinic, Ruhr University Bochum, Bochum, and Justus Liebig University, Giessen, Germany - Pathobiology 2006; 73:50-54. Here is an excerpt: "Abstract - Objective: Malignant mesothelioma of the peritoneum, pleura and pericardium can be easily confused with either metastatic adenocarcinoma or reactive pleural lesions. D2-40, a monoclonal antibody that is used as a marker for seminomatous germ cell tumors and lymphatic endothelial cells , recently described in mesothelial cells and type I but not type II pneumocytes. Methods: D2-40 immunoreactivities in 76 lung carcinomas of different histological types (adenocarcinoma, squamous cell, small cell, and bronchioloalveolar carcinoma) compared with those of epithelioid and sarcomatoid mesotheliomas 36 pleural and 5 specimens of chronic pleuritis. Results: While all 18 analyzed epithelioid mesotheliomas displayed a strong membrane immunostaining, 18 sarcomatoid mesotheliomas showed no signal, or only faint cytoplasm, comparable with fibroblasts in chronic pleurisy. From all lung carcinomas analyzed, 49 did not show immunoreactivity for D2-40 (64%), while 27 others (36%) showed focal and weak to moderate cytoplasmic signal only. Conclusions: We consider D2-40 as a valid marker in the differential diagnosis of epithelioid mesothelioma compared to lung adenocarcinoma. however, this marker can not be true sarcomatoid mesotheliomas label or distinguish them from reactive pleural lesions. "
We are all indebted to the researchers either. If you find one interesting quote, please see the study as a whole.
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