One interesting study is called, "turning adenovirus-mediated overexpression of wild-type p53 tumourigenicity human mesothelioma cells." With Giuliano M, A Catalano, Strizzi L, Vianale G, Capogrossi M, Procopio A. Int J Mol Med. June 2000, 5 (6) :591-6. Department of Oncology and Neuroscience, Section of Clinical Pathology, Gabriele D'Annunzio University, 66013 Chieti, Italy. Here is an excerpt: "Abstract - Malignant pleural mesothelioma (MM) showed poor survival, regardless of tumor stage at diagnosis. MM unresponsive to present treatment regimens and new protocols are needed. Nature of local, potential accessibility, and the relative lack of distant metastases make MM very attractive candidate for somatic gene therapy. A common target for cancer therapy is the tumor suppressor gene p53 protein. p53 does not seem to be mutated or deleted in MM, but can be inactivated by binding to other proteins, such as MDM2 and SV40 large T antigen. We tested effects of replication-deficient adenoviral vector carrying wild-type p53 cDNA in human MM cells. Our results indicate that> 95% of MM cells are efficiently infected with 25 multiplicity of infection (MOI) of the vector. Wild type p53 effectively disclosed resulted in inhibition of> 80 % proliferation in MM cells. AdCMV.p53 infection induced apoptosis while controls showed no obvious morphological changes. Ex vivo experiments p53 gene transfer inhibits tumor formation in nude mice. In vivo, direct injection AdCMV.p53 intratumour arrested tumor growth and prolonged survival of mice . results suggest that p53-gene therapy should be strongly utilized for clinical trials in patients with MM. "
Another study is called, "Congenital polycystic tumor atrioventricular node (endodermal heterotopia, mesothelioma): a histogenetic appraisal with evidence of its endodermal origin" - Human Pathology Volume 18, Issue 8, August 1987, Pages 791-795 by Gerald Both MD and MD Usha Raju . Here is an excerpt: "The small, various appointed, atrioventricular node tumor has been considered of primary endothelial endodermal origin, or mesothelial. To identify the derivatives, we studied seven tumors using silver staining and immunocytochemical labeling with various antibodies. Cytoplasmic granules argyrophil but not argentaffin granules found in the cells that are isolated in the cell layer Bubule-more in four tumors. Serotonin and calcitonin demonstrated tumor in seven and six, respectively, in a similar distribution of argyrophil cells. positive reaction distribution is different from the cell argyrophil cells recorded in various amounts tubule-lining cells for carcinoembryonic antigen, epithelial membrane antigen, and blood group antigens in seven, four and seven tumors, respectively. recorded no activity in tumor cells for factor VIII-related antigen or a peptide. than mesothelial or endodermal origin for the epithelial tumor amplified by the presence of neuroendocrine cells in the center of carcinoembryonic antigen-positive cells lining the tubules more tumors. "
Another study is called, "the expression of SV40 in tissue neoplastic and non-neoplastic human: perspectives on diagnosis, prognosis and therapy of human malignant mesothelioma." With Procopio A, Marinacci R, MR Marinetti, Strizzi L, Paludi D, Iezzi T, Tassi G, Casalini A, Modesti A. Dev Biol Stand. , 1998; 94:361-7. Department of Oncology and Neuroscience, Gabriele D'Annunzio University, Chieti, Italy. Here is an excerpt: "Abstract - We have demonstrated the association of SV40 and human malignant pleural mesothelioma. Here, we have investigated whether SV40 viral sequences may be associated with other human tumors or non-neoplastic pathology and whether SV40 DNA or protein expression may be diagnostic, relevance prognostic or therapeutic. DNA extracted from paraffin-embedded tissue. SV40, JC and BK virus sequences were detected by polymerase chain reaction and hybridization with probes specific molecules. Playback with three different sets of primers linked SV40 showed that 7/18 (38.8 %) mesothelioma SV40 positive specimens and 5/18 (27.7%) lesions of pleural tuberculosis. None of the 18 lung cancer, as well as 20 non-specific inflammatory pleural specimens tested positive. twenty-five blood samples and 18 sediment urine of MM patients were also negative. We have also found that the SV40 Tag protein present in mesothelioma cells and tumors. Tags proteins can interfere with tumor suppressor gene products, such as p53. Preliminary results showed that wild-type p53 transgene expression, obtained after recombinant adenovirus infection (AdCMV.p53), inhibits in vitro and in vivo proliferation, induces apoptosis of mesothelioma cells. Infection with the virus control is not effective. Accordingly, DNA and SV40 Tag expression in mesothelioma tumor cells, although it may not be relevant for diagnostic or prognostic purposes, may important for the strategy of innovative gene therapies. "
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